Sex after all: high levels of diversity detected in the arctic clonal plant Saxifraga cernua using RAPD markers
Identifieur interne : 002810 ( Main/Exploration ); précédent : 002809; suivant : 002811Sex after all: high levels of diversity detected in the arctic clonal plant Saxifraga cernua using RAPD markers
Auteurs : T. M. Gabrielsen [Norvège] ; C. Brochmann [Norvège]Source :
- Molecular Ecology [ 0962-1083 ] ; 1998-12.
Descripteurs français
- Wicri :
- topic : Arctique.
English descriptors
- KwdEn :
Abstract
Arctic plants in general and arctic clonal plants in particular have often been assumed to contain low levels of genetic diversity. We used RAPDs (random amplified polymorphic DNAs) to investigate genetic diversity in the arctic‐alpine Saxifraga cernua, which mainly reproduces clonally via bulbils, at three spatial scales in Svalbard: (i) ‘macroscale’, between two sites 11 km apart; (ii) ‘mesoscale’, along two crossing transects at each site; and (iii) ‘microscale’, within a 3 × 3 m square at each site. Thirteen putative clones (RAPD phenotypes) were distinguished among 93 ramets based on 38 RAPD markers. The genetic diversity (D; mean 0.52, range 0.10–0.81) and evenness (E; mean 0.42, range 0.00–0.82) were at the same level as in clonal plants in general. However, the diversity strongly depended on site and spatial scale. Several clones were highly divergent and clustered independently of site in UPGMA and PCO analyses. In an analysis of molecular variance (AMOVA), most of the variation (59%) was found within sites. Mantel tests revealed no correlation between spatial and genetic distance within sites. Our results suggest that occasional sexual reproduction as well as clonal migration via bulbil dispersal play a significant role in the treeless arctic environment, where S. cernua is widespread and locally very abundant. In contrast, Bauert et al. (Molecular Ecology 7, 1519–1527) found no genetic variation within populations or regions of the Alps, where the species has highly isolated occurrences.
Url:
DOI: 10.1046/j.1365-294x.1998.00503.x
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000D58
- to stream Istex, to step Curation: 000D58
- to stream Istex, to step Checkpoint: 000D20
- to stream Main, to step Merge: 002927
- to stream Main, to step Curation: 002810
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Sex after all: high levels of diversity detected in the arctic clonal plant Saxifraga cernua using RAPD markers</title><author><name sortKey="Gabrielsen, T M" sort="Gabrielsen, T M" uniqKey="Gabrielsen T" first="T. M." last="Gabrielsen">T. M. Gabrielsen</name></author><author><name sortKey="Brochmann, C" sort="Brochmann, C" uniqKey="Brochmann C" first="C." last="Brochmann">C. Brochmann</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:F31E0ECFC1869BDC8DE87F4C69579D632ADA3367</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1046/j.1365-294x.1998.00503.x</idno><idno type="url">https://api.istex.fr/document/F31E0ECFC1869BDC8DE87F4C69579D632ADA3367/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000D58</idno><idno type="wicri:Area/Istex/Curation">000D58</idno><idno type="wicri:Area/Istex/Checkpoint">000D20</idno><idno type="wicri:doubleKey">0962-1083:1998:Gabrielsen T:sex:after:all</idno><idno type="wicri:Area/Main/Merge">002927</idno><idno type="wicri:Area/Main/Curation">002810</idno><idno type="wicri:Area/Main/Exploration">002810</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Sex after all: high levels of diversity detected in the arctic clonal plant Saxifraga cernua using RAPD markers</title><author><name sortKey="Gabrielsen, T M" sort="Gabrielsen, T M" uniqKey="Gabrielsen T" first="T. M." last="Gabrielsen">T. M. Gabrielsen</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Botanical Garden and Museum, University of Oslo, Trondheimsveien 23 B, N‐0562 Oslo</wicri:regionArea><wicri:noRegion>N‐0562 Oslo</wicri:noRegion></affiliation></author><author><name sortKey="Brochmann, C" sort="Brochmann, C" uniqKey="Brochmann C" first="C." last="Brochmann">C. Brochmann</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Botanical Garden and Museum, University of Oslo, Trondheimsveien 23 B, N‐0562 Oslo</wicri:regionArea><wicri:noRegion>N‐0562 Oslo</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Ecology</title><idno type="ISSN">0962-1083</idno><idno type="eISSN">1365-294X</idno><imprint><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1998-12">1998-12</date><biblScope unit="volume">7</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="1701">1701</biblScope><biblScope unit="page" to="1708">1708</biblScope></imprint><idno type="ISSN">0962-1083</idno></series><idno type="istex">F31E0ECFC1869BDC8DE87F4C69579D632ADA3367</idno><idno type="DOI">10.1046/j.1365-294x.1998.00503.x</idno><idno type="ArticleID">MEC503</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0962-1083</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arctic</term><term>RAPDs</term><term>Saxifraga cernua</term><term>clonal plants</term><term>genetic diversity</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Arctique</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Arctic plants in general and arctic clonal plants in particular have often been assumed to contain low levels of genetic diversity. We used RAPDs (random amplified polymorphic DNAs) to investigate genetic diversity in the arctic‐alpine Saxifraga cernua, which mainly reproduces clonally via bulbils, at three spatial scales in Svalbard: (i) ‘macroscale’, between two sites 11 km apart; (ii) ‘mesoscale’, along two crossing transects at each site; and (iii) ‘microscale’, within a 3 × 3 m square at each site. Thirteen putative clones (RAPD phenotypes) were distinguished among 93 ramets based on 38 RAPD markers. The genetic diversity (D; mean 0.52, range 0.10–0.81) and evenness (E; mean 0.42, range 0.00–0.82) were at the same level as in clonal plants in general. However, the diversity strongly depended on site and spatial scale. Several clones were highly divergent and clustered independently of site in UPGMA and PCO analyses. In an analysis of molecular variance (AMOVA), most of the variation (59%) was found within sites. Mantel tests revealed no correlation between spatial and genetic distance within sites. Our results suggest that occasional sexual reproduction as well as clonal migration via bulbil dispersal play a significant role in the treeless arctic environment, where S. cernua is widespread and locally very abundant. In contrast, Bauert et al. (Molecular Ecology 7, 1519–1527) found no genetic variation within populations or regions of the Alps, where the species has highly isolated occurrences.</div></front></TEI><affiliations><list><country><li>Norvège</li></country></list><tree><country name="Norvège"><noRegion><name sortKey="Gabrielsen, T M" sort="Gabrielsen, T M" uniqKey="Gabrielsen T" first="T. M." last="Gabrielsen">T. M. Gabrielsen</name></noRegion><name sortKey="Brochmann, C" sort="Brochmann, C" uniqKey="Brochmann C" first="C." last="Brochmann">C. Brochmann</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/OperaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002810 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002810 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= OperaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:F31E0ECFC1869BDC8DE87F4C69579D632ADA3367
|texte= Sex after all: high levels of diversity detected in the arctic clonal plant Saxifraga cernua using RAPD markers
}}
| This area was generated with Dilib version V0.6.21. |  |